af488 donkey α-rabbit igg  (Thermo Fisher)

Journal: Cells

Article Title: An Optimized Protocol for the Generation of Alveolospheres from Wild-Type Mice

doi: 10.3390/cells13110922

Figure Lengend Snippet: List of antibodies used for immunofluorescence.

Article Snippet: Rabbit α-Pro-SFTPC (Seven Hills Bioreagents, WRAB-9337, Cincinnati, OH, USA) , 1:800 , Donkey α-rabbit AF488 (Thermo Fisher Scientific, A-32790, Waltham, MA, USA) , 1:1000.

Techniques: Immunofluorescence

Journal: eLife

Article Title: Metamorphosis of memory circuits in Drosophila reveals a strategy for evolving a larval brain

doi: 10.7554/eLife.80594

Figure Lengend Snippet: Reagents used in this study.

Article Snippet: AF488 donkey α-rabbit , Jackson ImmunoResearch , #711-545-152.

Techniques: Electron Microscopy

Journal: PLoS ONE

Article Title: Inducible Gene Manipulations in Brain Serotonergic Neurons of Transgenic Rats

doi: 10.1371/journal.pone.0028283

Figure Lengend Snippet: (A) Mouse TPH2-CreERT2 expression cassette for DNA microinjection. (B–I) DAB-immunohistochemistry with a Cre antibody shows weak Cre expression in the brain stem and mid-brain of TPH2-CreERT2 rat founder lines #7 ( B,C), #8 ( D,E), and #14 ( F,G). Founder line #15 shows extensive Cre staining in areas where serotonergic raphe nuclei are located ( H,I). Intensity of Cre expression correlates with the transgene copy number of TPH2-CreERT2 rat founders ( J).

Article Snippet: Secondary antibodies were AF488 donkey α-rabbit (Invitrogen, 1∶1000 for TPH2 and 1∶5000 for GFP and Cre), Cy3 donkey α-mouse (Jackson ImmunoResearch, 1∶200 for TPH1), Cy3 donkey α-chicken (Jackson ImmunoResearch, 1∶1000 for βgalactosidase) and AF488 donkey α-mouse (Invitrogen, 1∶200 for GAD67, NeuN, TH and GFAP).

Techniques: Expressing, Immunohistochemistry, Staining

Journal: PLoS ONE

Article Title: Inducible Gene Manipulations in Brain Serotonergic Neurons of Transgenic Rats

doi: 10.1371/journal.pone.0028283

Figure Lengend Snippet: (A,C,E,G) DAB-immunohistochemistry with a Cre antibody of line #15 shows Cre staining in the brain stem and mid-brain, regions which contain serotonergic somata while extraserotonergic brain regions show no staining. (B,D,F,H) Coronal sections of dual-label fluorescence immunohistochemistry with Cre and TPH1 antibodies. The TPH1 antibody crossreacts with TPH2 and detects both isoenzymes. Colocalization of TPH1 and Cre confirms exclusive Cre expression in 5-HT neurons of the raphe nuclei. Caudal raphe nuclei (CR); dorsal raphe nuclei (DR); median raphe nuclei (MR). Scale bars: 100 µm.

Article Snippet: Secondary antibodies were AF488 donkey α-rabbit (Invitrogen, 1∶1000 for TPH2 and 1∶5000 for GFP and Cre), Cy3 donkey α-mouse (Jackson ImmunoResearch, 1∶200 for TPH1), Cy3 donkey α-chicken (Jackson ImmunoResearch, 1∶1000 for βgalactosidase) and AF488 donkey α-mouse (Invitrogen, 1∶200 for GAD67, NeuN, TH and GFAP).

Techniques: Immunohistochemistry, Staining, Fluorescence, Expressing

Journal: PLoS ONE

Article Title: Inducible Gene Manipulations in Brain Serotonergic Neurons of Transgenic Rats

doi: 10.1371/journal.pone.0028283

Figure Lengend Snippet: (A,B) X-Gal staining of sagittal sections shows ubiquitous βgal activity throughout the brain of adult CAG-loxP.EGFP rats (P90). (C–K) Dual-label fluorescence immunohistochemistry (IHC). (C–E) βgal/NeuN IHC of the cerebellum (C), cortex (D) and OB (E) shows strong colocalization of βgal with the neuronal marker NeuN. (F–H) βgal IHC with the serotonergic marker TPH2 (F), and the dopaminergic and noradrenergic marker tyrosine hydroxylase (TH) (G,H) shows abundant colocalization of βgal with 5-HT neurons in the dorsal raphe (F), with dopaminergic neurons in the ventral tegmental area and substantia nigra (G) and noradrenergic neurons in the locus coeruleus (H) confirming strong βgal expression in all monoaminergic neurons. (I,J) βgal/GAD67 IHC shows βgal expression in GABAergic neurons of the granular layer of the OB (I) and in the hippocampus (J). (K) βgal/GFAP IHC in the hippocampus shows infrequent βgal expression in glia. OB, olfactory bulb; DR, dorsal raphe nuclei; VTA, ventral tegmental area; SN, substantia nigra; LC, locus coeruleus; HC, hippocampus. Scale bars: 100 µm.

Article Snippet: Secondary antibodies were AF488 donkey α-rabbit (Invitrogen, 1∶1000 for TPH2 and 1∶5000 for GFP and Cre), Cy3 donkey α-mouse (Jackson ImmunoResearch, 1∶200 for TPH1), Cy3 donkey α-chicken (Jackson ImmunoResearch, 1∶1000 for βgalactosidase) and AF488 donkey α-mouse (Invitrogen, 1∶200 for GAD67, NeuN, TH and GFAP).

Techniques: Staining, Activity Assay, Fluorescence, Immunohistochemistry, Marker, Expressing

Journal: PLoS ONE

Article Title: Inducible Gene Manipulations in Brain Serotonergic Neurons of Transgenic Rats

doi: 10.1371/journal.pone.0028283

Figure Lengend Snippet: (A) TPH2-CreERT2 rats were bred to CAG-loxP.EGFP rats to generate double-transgenic TPH2-CreERT2/CAG-loxP.EGFP rats. Under uninduced baseline conditions, the loxP-flanked lacZ minigene is expressed reflecting cell-type specific CAG-promoter activity. Upon Cre-mediated recombination (+ Tamoxifen), lacZ is replaced with the second reporter gene enhanced green fluorescent protein (EGFP). The appearance of EGFP serves as an indicator of Cre mediated recombination in double transgenic rats. TPH2-CreERT2/CAG-loxP.EGFP rats were daily injected with tamoxifen (40 mg/kg) or vehicle for five consecutive days starting between P60–90. Coronal sections show dual-label fluorescence immunohistochemistry for TPH/βgal (B,E,H,K) and TPH/GFP (C,F,I,L) in vehicle-treated rats (-Tx) and TPH/GFP in tamoxifen-treated (+Tx) rats (D,G,J,M). Colocalization is visualized at the level of caudal raphe nuclei (CR) (B–D), dorsal raphe nuclei (DR) (E–J) and median raphe nuclei (MR) (K–M) using confocal images. In vehicle-treated rats, TPH2-CreERT2/CAG-loxP.EGFP rats display strong basal, non-recombined βgal expression in TPH2+ 5-HT neurons (B,E,H,K) making these rats ideally suited to monitor tamoxifen-induced Cre-mediated recombination in 5-HT neurons. (C,F,I,L) Without tamoxifen treatment, background recombination, i.e. EGFP expression (arrows) hardly occurs. (D,G,J,M) After tamoxifen treatment, the majority of TPH+ 5-HT neurons in all raphe nuclei now show EGFP expression indicating Cre-mediated recombination in these neurons (GFP+/TPH+). Scale bars: 100 µm.

Article Snippet: Secondary antibodies were AF488 donkey α-rabbit (Invitrogen, 1∶1000 for TPH2 and 1∶5000 for GFP and Cre), Cy3 donkey α-mouse (Jackson ImmunoResearch, 1∶200 for TPH1), Cy3 donkey α-chicken (Jackson ImmunoResearch, 1∶1000 for βgalactosidase) and AF488 donkey α-mouse (Invitrogen, 1∶200 for GAD67, NeuN, TH and GFAP).

Techniques: Transgenic Assay, Activity Assay, Injection, Fluorescence, Immunohistochemistry, Expressing

Journal: PLoS ONE

Article Title: Inducible Gene Manipulations in Brain Serotonergic Neurons of Transgenic Rats

doi: 10.1371/journal.pone.0028283

Figure Lengend Snippet: Recombination efficacy and background recombination for rat TPH2-CreERT2 line #15.

Article Snippet: Secondary antibodies were AF488 donkey α-rabbit (Invitrogen, 1∶1000 for TPH2 and 1∶5000 for GFP and Cre), Cy3 donkey α-mouse (Jackson ImmunoResearch, 1∶200 for TPH1), Cy3 donkey α-chicken (Jackson ImmunoResearch, 1∶1000 for βgalactosidase) and AF488 donkey α-mouse (Invitrogen, 1∶200 for GAD67, NeuN, TH and GFAP).

Techniques: